ELISpot Flex: Human IL-1α (ALP)

3414-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT641/642
Detection mAb	MT513, biotin
Enzyme conjugate	Streptavidin-ALP

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Delivery 4-9 business days

Shipping $0

Complementary products

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ELISpot substrate: BCIP/NBT-plus for ALP

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-1α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Human IL-1α (ALP)
Application	ELISpot
Analyte	IL-1α
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-1α (pro-form and active form).
Cross-reactivity	The assay shows no cross-reactivity with recombinant human IL-1β or IL-18.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Performance

Human PBMC were incubated for 20 hours in an ELISpot plate. Cells were unstimulated (20,000 cells/well) or stimulated with LPS (5000 cells/well). The number of cells secreting IL-1α was analyzed by ELISpot.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (0)

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Analyte information

IL-1α

Analyte description	Interleukin 1α (IL-1α) is a pro-inflammatory cytokine primarily produced by monocytes, macrophages, and neutrophils. IL-1α induces acute phase responses such as fever, T cell, and macrophage activation.
Alternative names	Interleukin 1α, IL-1α, hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α, Interleukin-1 alpha, IL-1A, IL1, IL1-ALPHA, IL1F1
Cell type	Monocyte/MΦ, mDC
Gene ID	3552